Coding

Part:BBa_K3890006:Design

Designed by: Eric Andre Velasco Yepez   Group: iGEM21_USP-Brazil   (2021-10-20)


CYP6G1 CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1416
    Illegal SapI site found at 1246


Design Notes

This coding sequence was synthesized and is codon optimized for Solanum lycopersicum, in order to be used in plants system expression. It was originally identified in Drosophila melanogaster (fruit fly) [1]. Additionally, the stop codon sequence was removed to allow the fusion with the LP4/2A self-cleavage peptide in order to get a polycistronic expression in eukaryotes organisms. This part is also used in BBa_K3890000 composite part.

Source

The CDS of cyp6g1 from Drosophila melanogaster genome was obtained by GenBank in NCBI.

References

[1] Joußen, Nicole, et al. "Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1‐overexpressing strains of Drosophila melanogaster, leading to resistance." Pest management science 64.1 (2008): 65-73.